https://ephjournal.com/index.php/bps/issue/feed EPH - International Journal of Biological & Pharmaceutical Science (ISSN: 2208-2166) 2019-05-14T06:00:06+00:00 Naveen Malik editor@ephjournal.com Open Journal Systems <p><strong><span id="cell-3-name" class="gridCellContainer"><span class="label">EPH - International Journal of Biological &amp; Pharmaceutical Science (ISSN: 2208-2166) </span></span></strong> publishes a wide range of high quality research articles in the field (but not limited to) given below: biological sciences. Scope of the journal includes: Cell biology, developmental biology, structural biology, microbiology, molecular biology &amp; genetics, biochemistry, biotechnology, biodiversity, entomology, toxicology, ecology, freshwater biology, marine biology etc. <br><span style="font-size: 1.5em;"><strong> <span style="text-shadow: #ff6600 0px 0px 3px;">Current Impact Factor: 2.448</span></strong></span></p> https://ephjournal.com/index.php/bps/article/view/1186 Hydrogen peroxide impedes NK cell and T cell mediated cytotoxicity against colon cancer cell 2019-05-14T06:00:06+00:00 SELLAMUTHU SUBBANNA GOUNDER ssellamuthu@gmail.com Baskar Subramani baskar@niscell.com Chithra Ramanathan Pullai chithra@niscell.com Ezzati Radzuan ezzaati@nicell.com Arulvasu Chinnasamy carulvasu@niscell.com <p>Although hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is an admirable treatment for many cancer types, but the clinical impact of H<sub>2</sub>O<sub>2 </sub>on immune cells is not known. In the present study, we have demonstrated that H<sub>2</sub>O<sub>2</sub> is a crucial factor to determine the cell mediated cytotoxicity of immune cells to the cancer cells. Natural killer (NK) cells and T cells were isolated from healthy donors and were activated and enriched the population at <em>ex-vivo</em> condition. At day14, activated NK and T cells were exposed to colon cancer cell at the ratio of 10:1 (Effector: Target) with or without H<sub>2</sub>O<sub>2</sub> (50 µM and 100µM) treatment for 6 h and 12 h. Cytotoxicity assay showed that cytotolytic ability of NK and T cells was strongly suppressed in the presence of H<sub>2</sub>O<sub>2</sub>. H<sub>2</sub>O<sub>2 </sub>induced NK and T cell dysfunction was analyzed and results demonstrated that NK and T cells were lose their viability on dose and time dependent manner. Also, flow cytometry analysis revealed that H<sub>2</sub>O<sub>2 </sub>significantly suppresses the activation and proliferation CD8 T cell population. Whereas, NK cell subset (CD56<sup>bright</sup> and CD56<sup>dim</sup>) was dramatically altered by H<sub>2</sub>O<sub>2</sub>. When exposed to H<sub>2</sub>O<sub>2</sub>, CD56<sup>dim</sup> population was considerably increased at different time points. &nbsp;Hence, we conclude that H<sub>2</sub>O<sub>2 </sub>directly or functionally defect the NK and T cell mediated cytotoxicity and however, further studies are warranted to confirm the concept with mild dose of H<sub>2</sub>O<sub>2 </sub>with different cancer cell lines.&nbsp; &nbsp;&nbsp;&nbsp;</p> 2019-04-28T07:09:21+00:00 Copyright (c) 2019 EPH - International Journal of Biological & Pharmaceutical Science (ISSN: 2208-2166)