EPH - International Journal of Biological & Pharmaceutical Science (ISSN: 2208-2166) https://ephjournal.com/index.php/bps <p><strong><span id="cell-3-name" class="gridCellContainer"><span class="label">EPH - International Journal of Biological &amp; Pharmaceutical Science (ISSN: 2208-2166) </span></span></strong> publishes a wide range of high quality research articles in the field (but not limited to) given below: biological sciences. Scope of the journal includes: Cell biology, developmental biology, structural biology, microbiology, molecular biology &amp; genetics, biochemistry, biotechnology, biodiversity, entomology, toxicology, ecology, freshwater biology, marine biology etc. <br><span style="font-size: 1.5em;"><strong> <span style="text-shadow: #ff6600 0px 0px 3px;">Current Impact Factor: 2.448</span></strong></span></p> EPH Journal en-US EPH - International Journal of Biological & Pharmaceutical Science (ISSN: 2208-2166) 2208-2166 <ul> <li>All contributor(s) agree to transfer the copyright of this article to <strong>EPH Journal.</strong></li> <li><strong>EPH Journal</strong> will have all the rights to distribute, share, sell, modify this research article with proper reference of the contributors.&nbsp;</li> <li><strong>EPH Journal</strong> will have the right to edit or completely remove the published article on any misconduct happening.</li> </ul> Hydrogen peroxide impedes NK cell and T cell mediated cytotoxicity against colon cancer cell https://ephjournal.com/index.php/bps/article/view/1186 <p>Although hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is an admirable treatment for many cancer types, but the clinical impact of H<sub>2</sub>O<sub>2 </sub>on immune cells is not known. In the present study, we have demonstrated that H<sub>2</sub>O<sub>2</sub> is a crucial factor to determine the cell mediated cytotoxicity of immune cells to the cancer cells. Natural killer (NK) cells and T cells were isolated from healthy donors and were activated and enriched the population at <em>ex-vivo</em> condition. At day14, activated NK and T cells were exposed to colon cancer cell at the ratio of 10:1 (Effector: Target) with or without H<sub>2</sub>O<sub>2</sub> (50 µM and 100µM) treatment for 6 h and 12 h. Cytotoxicity assay showed that cytotolytic ability of NK and T cells was strongly suppressed in the presence of H<sub>2</sub>O<sub>2</sub>. H<sub>2</sub>O<sub>2 </sub>induced NK and T cell dysfunction was analyzed and results demonstrated that NK and T cells were lose their viability on dose and time dependent manner. Also, flow cytometry analysis revealed that H<sub>2</sub>O<sub>2 </sub>significantly suppresses the activation and proliferation CD8 T cell population. Whereas, NK cell subset (CD56<sup>bright</sup> and CD56<sup>dim</sup>) was dramatically altered by H<sub>2</sub>O<sub>2</sub>. When exposed to H<sub>2</sub>O<sub>2</sub>, CD56<sup>dim</sup> population was considerably increased at different time points. &nbsp;Hence, we conclude that H<sub>2</sub>O<sub>2 </sub>directly or functionally defect the NK and T cell mediated cytotoxicity and however, further studies are warranted to confirm the concept with mild dose of H<sub>2</sub>O<sub>2 </sub>with different cancer cell lines.&nbsp; &nbsp;&nbsp;&nbsp;</p> SELLAMUTHU SUBBANNA GOUNDER Baskar Subramani Chithra Ramanathan Pullai Ezzati Radzuan Arulvasu Chinnasamy Copyright (c) 2019 EPH - International Journal of Biological & Pharmaceutical Science (ISSN: 2208-2166) http://creativecommons.org/licenses/by-nc-nd/4.0 2019-04-28 2019-04-28 5 4 01 14